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Donnellan, Samantha, Pennington, Shaun 
ORCID: https://orcid.org/0000-0002-7160-6275, Ruggiero, Alessandra, Martinez-Rodriguez, Carmen, Pouget, Marion, Thomas, Jordan, Ward, Steve ORCID: https://orcid.org/0000-0003-2331-3192, Pollakis, Georgios, Biagini, Giancarlo ORCID: https://orcid.org/0000-0001-6356-6595 and Paxton, William
(2022)
'A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection'. Journal of Infectious Disease, Vol 227, Issue 5, pp. 708-713.
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Abstract
M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.
| Item Type: | Article |
|---|---|
| Subjects: | WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503 Acquired immunodeficiency syndrome. HIV infections WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503.5 Complications WF Respiratory System > Tuberculosis > WF 200 Tuberculosis (General) |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1093/infdis/jiac491 |
| Depositing User: | Marie Hatton |
| Date Deposited: | 12 Jan 2023 14:50 |
| Last Modified: | 04 Apr 2023 14:26 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/21746 |
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