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Hegde, Shivanand, Brettell, Laura, Quek, Shannon 
ORCID: https://orcid.org/0000-0002-1583-9541, Etebari, Kayvan, Saldaña, Miguel A., Asgari, Sassan, Coon, Kerri L., Heinz, Eva ORCID: https://orcid.org/0000-0003-4413-3756 and Hughes, Grant ORCID: https://orcid.org/0000-0002-7567-7185
(2024)
'Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes'. Environmental Microbiology, Vol 26, Issue 2, e16576.
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1462-2920.16576.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito species, life stages, and geography. How the host controls and is affected by this variation is unclear. Using microbiome transplant experiments, we asked whether there were differences in transcriptional responses when mosquitoes of different species were used as microbiome donors. We used microbiomes from four different donor species spanning the phylogenetic breadth of the Culicidae, collected either from the laboratory or the field. We found that when recipients received a microbiome from a donor reared in the laboratory, the response was remarkably similar regardless of donor species. However, when the donor had been collected from the field, many more genes were differentially expressed. We also found that while the transplant procedure did have some effect on the host transcriptome, this is likely to have had a limited effect on mosquito fitness. Overall, our results highlight the possibility that variation in mosquito microbiome communities is associated with variability in host–microbiome interactions and further demonstrate the utility of the microbiome transplantation technique for investigating host–microbe interactions in mosquitoes.
| Item Type: | Article | ||||
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| Subjects: | QW Microbiology and Immunology > QW 4 General works. Classify here works on microbiology as a whole. QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 525 Aedes |
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| Faculty: Department: | Biological Sciences > Vector Biology Department | ||||
| Digital Object Identifer (DOI): | https://doi.org/10.1111/1462-2920.16576 | ||||
| SWORD Depositor: | JISC Pubrouter | ||||
| Depositing User: | JISC Pubrouter | ||||
| Date Deposited: | 17 Jan 2024 16:09 | ||||
| Last Modified: | 27 Feb 2024 13:33 | ||||
| URI: | https://archive.lstmed.ac.uk/id/eprint/23807 |
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