Tools
Tools
Bell-Sakyi, Lesley, Haines, Lee 
ORCID: https://orcid.org/0000-0001-8821-6479, Petrucci, Giovanni, Beliavskaia, Alexandra, Hartley, Catherine, Khoo, Jing Jing, Makepeace, Benjamin L., Abd-Alla, Adly M. M. and Darby, Alistair C.
(2024)
'Establishment and partial characterisation of a new cell line derived from adult tissues of the tsetse fly Glossina morsitans morsitans'. Parasites & Vectors, Vol 17, Issue 1, e231.
|
Text
13071_2024_Article_6310.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Background: Insect cell lines play a vital role in many aspects of research on disease vectors and agricultural pests. The tsetse fly Glossina morsitans morsitans is an important vector of salivarian trypanosomes in sub-Saharan Africa and, as such, is a major constraint on human health and agricultural development in the region.
Methods: Here, we report establishment and partial characterisation of a cell line, GMA/LULS61, derived from tissues of adult female G. m. morsitans. GMA/LULS61 cells, grown at 28 °C in L-15 (Leibovitz) medium supplemented with foetal bovine serum and tryptose phosphate broth, have been taken through 23 passages to date and can be split 1:1 at 2-week intervals. Karyotyping at passage 17 revealed a predominantly haploid chromosome complement. Species origin and absence of contaminating bacteria were confirmed by PCR amplification and sequencing of fragments of the COI gene and pan-bacterial 16S rRNA gene respectively. However, PCR screening of RNA extracted from GMA/LULS61 cells confirmed presence of the recently described Glossina morsitans morsitans iflavirus and Glossina morsitans morsitans negevirus, but absence of Glossina pallipides salivary gland hypertrophy virus. GMA/LULS61 cells supported infection and growth of 6/7 different insect-derived strains of the intracellular bacterial symbiont Wolbachia.
Conclusions: The GMA/LULS61 cell line has potential for application in a variety of studies investigating the biology of G. m. morsitans and its associated pathogenic and symbiotic microorganisms.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > Cells and Genetics > QU 350 Cellular structures QX Parasitology > QX 20 Research (General) QX Parasitology > Insects. Other Parasites > QX 505 Diptera |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1186/s13071-024-06310-9 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 23 May 2024 08:58 |
| Last Modified: | 23 May 2024 08:58 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/24582 |
Statistics
Actions (login required)
 |
Edit Item |