Single-cell-resolved interspecies comparison shows a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19

Peidli, Stefan, Nouailles, Geraldine, Wyler, Emanuel, Adler, Julia M., Kunder, Sandra, Voß, Anne, Kazmierski, Julia, Pott, Fabian, Pennitz, Peter, Postmus, Dylan, Teixeira Alves, Luiz Gustavo, Goffinet, Christine, Gruber, Achim D., Blüthgen, Nils, Witzenrath, Martin, Trimpert, Jakob, Landthaler, Markus and Praktiknjo, Samantha D. (2024) 'Single-cell-resolved interspecies comparison shows a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19'. Cell Reports, Vol 43, Issue 6, e114328.

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Abstract

A key issue for research on COVID-19 pathogenesis is the lack of biopsies from patients and of samples at the onset of infection. To overcome these hurdles, hamsters were shown to be useful models for studying this disease. Here, we further leverage the model to molecularly survey the disease progression from time-resolved single-cell RNA sequencing data collected from healthy and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected Syrian and Roborovski hamster lungs. We compare our data to human COVID-19 studies, including bronchoalveolar lavage, nasal swab, and postmortem lung tissue, and identify a shared axis of inflammation dominated by macrophages, neutrophils, and endothelial cells, which we show to be transient in Syrian and terminal in Roborovski hamsters. Our data suggest that, following SARS-CoV-2 infection, commitment to a type 1- or type 3-biased immunity determines moderate versus severe COVID-19 outcomes, respectively.

Item Type:	Article
Uncontrolled Keywords:	NOT_LSTM
Subjects:	QU Biochemistry > Cells and Genetics > QU 350 Cellular structures WC Communicable Diseases > WC 20 Research (General) WC Communicable Diseases > Virus Diseases > Viral Respiratory Tract Infections. Respirovirus Infections > WC 506 COVID-19
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.celrep.2024.114328
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	25 Jun 2024 12:12
Last Modified:	25 Jun 2024 12:13
URI:	https://archive.lstmed.ac.uk/id/eprint/24822

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