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The conserved structure of snake venom toxins confers extensive immunological cross-reactivity to toxin-specific antibody

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Harrison, Robert, Wuster, W. and Theakston, R.David G. (2003) 'The conserved structure of snake venom toxins confers extensive immunological cross-reactivity to toxin-specific antibody'. Toxicon, Vol 41, Issue 4, pp. 441-449.

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Official URL: http://www.journals.elsevier.com/toxicon/

Abstract

We have demonstrated previously that antisera from mice immunised with DNA encoding the carboxy-terminal domain (JD9) of a potent haemorrhagic metalloproteinase, jararhagin, neutralised over 70% of the haemorrhagic activity of the whole Bothrops jararaca venom. Here, we demonstrate that the JD9-specific antibody possesses extensive immunological reactivity to venom components in snakes of distinct species and genera. The polyspecific immunological reactivity of the antibody showed a correlation with amino acid sequence identity and with predicted antigenic domains of JD9-analogues in venoms of snakes with closest phylogenetic links to B. jararaca. This study further promotes the potential of DNA immunisation to generate toxin-specific antibodies with polyspecific cover. An analysis of the reactivity of the JD9-specific antisera to B. atrox complex venoms that exhibited intraspecific variation in the venom proteome revealed, however, that the toxin-specific approach to antivenom development requires a more in-depth knowledge of the target molecules than is required for conventional antivenoms.

Item Type:	Article
Subjects:	QV Pharmacology > Toxicology > General Toxicology > QV 601 Antidotes and other therapeutic measures QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 630 Toxins. Antitoxins QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 805 Vaccines. Antitoxins. Toxoids WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/s0041-0101(02)00360-4
Depositing User:	Users 476 not found.
Date Deposited:	06 Dec 2012 09:47
Last Modified:	06 Feb 2018 13:04
URI:	https://archive.lstmed.ac.uk/id/eprint/2564

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