Development of an oral regimen of unithiol for the treatment of snakebite envenoming: a phase 1 open-label dose-escalation safety trial and pharmacokinetic analysis in healthy Kenyan adults.

Abouyannis, Michael ORCID: https://orcid.org/0000-0003-4856-4334, Nyambura, Yvonne K, Ngome, Samson, Riako, Debra, Musyoki, Jennifer, Muiruri, Charles, Orindi, Benedict, Else, Laura, Amara, Alieu, Dickinson, Laura, Clare, Rachel ORCID: https://orcid.org/0000-0002-3945-0530, Albulescu, Laura-Oana ORCID: https://orcid.org/0000-0001-6563-9217, Westhorpe, Adam, Kool, Jeroen, Adetifa, Ifedayo, Ndungu, Francis M, FitzGerald, Richard, Khoo, Saye, Lalloo, David ORCID: https://orcid.org/0000-0001-7680-2200, Casewell, Nicholas ORCID: https://orcid.org/0000-0002-8035-4719 and Hamaluba, Mainga (2025) 'Development of an oral regimen of unithiol for the treatment of snakebite envenoming: a phase 1 open-label dose-escalation safety trial and pharmacokinetic analysis in healthy Kenyan adults.'. EBioMedicine, Vol 113, e105600.

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Abstract

Viperidae snakes are responsible for many of the 94,000 deaths caused by snakebite envenoming each year. The most pathological venom component of this globally diverse family of snakes are the zinc-dependent snake venom metalloproteinase (SVMP) enzymes, which can be inhibited by the metal chelator, unithiol. A short-course oral regimen, readily available and rapidly deployed ahead of hospital admission is needed. This open-label, phase 1 clinical trial assessed the safety of single ascending oral, multiple ascending oral, and single ascending intravenous doses of unithiol in 64 healthy adult volunteers from Kilifi County, Kenya. The multiple dose stage was informed by an interim safety and pharmacokinetic analysis, and predefined target plasma concentrations. Plasma concentrations of unithiol were measured using high-performance liquid chromatography-mass spectrometry, and safety was described by full adverse event reporting. 175 individuals were screened, and 64 (median age 30 years, IQR 25-38 years) received the study drug. There were no dose limiting toxicities or serious adverse events. There were 61 solicited adverse events, 17 related unsolicited adverse events, and 53 laboratory adverse events, all of mild or moderate severity. The maximum oral dose of 1500 mg was well tolerated and associated with the following pharmacokinetic parameters: C 14.7 μg/mL, T 2.9 h, T 18.4 h, and AUC 204.5 μg.h/mL. The phase 2 recommended dose (1500 mg loading dose, followed by 900 mg doses at 6-h and 24-h) has no safety concerns, and has promising pharmacokinetic properties for clinical use. Unithiol is affordable, stable at room temperature, and has the potential to be given orally in remote rural clinics. Its further development for snakebite indication is warranted.

Item Type:	Article
Subjects:	QV Pharmacology > QV 38 Drug action. QV Pharmacology > Toxicology > General Toxicology > QV 601 Antidotes and other therapeutic measures WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.ebiom.2025.105600
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	19 Mar 2025 14:39
Last Modified:	19 Mar 2025 14:39
URI:	https://archive.lstmed.ac.uk/id/eprint/26366

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