LSTM Home > LSTM Research > LSTM Online Archive

Loss of pH control in Plasmodium falciparum parasites subjected to oxidative stress

van Schalkwyk, Donelly A., Saliba, Kevin J., Biagini, Giancarlo ORCID: https://orcid.org/0000-0001-6356-6595, Bray, Patrick and Kirk, Kiaran (2013) 'Loss of pH control in Plasmodium falciparum parasites subjected to oxidative stress'. PLoS ONE, Vol 8, Issue 3, e58933.

[img]
Preview
Text
Plos_ONE_8_3_e58933.pdf - Published Version
Available under License Creative Commons Attribution.

Download (578kB)

Abstract

The intraerythrocytic malaria parasite is susceptible to oxidative stress and this may play a role in the mechanism of action of some antimalarial agents. Here we show that exposure of the intraerythrocytic malaria parasite to the oxidising agent hydrogen peroxide results in a fall in the intracellular ATP level and inhibition of the parasite's V-type H(+)-ATPase, causing a loss of pH control in both the parasite cytosol and the internal digestive vacuole. In contrast to the V-type H(+)-ATPase, the parasite's digestive vacuole H(+)-pyrophosphatase is insensitive to hydrogen peroxide-induced oxidative stress. This work provides insights into the effects of oxidative stress on the intraerythrocytic parasite, as well as providing an alternative possible explanation for a previous report that light-induced oxidative stress causes selective lysis of the parasite's digestive vacuole.

Item Type: Article
Subjects: QX Parasitology > Protozoa > QX 135 Plasmodia
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1371/journal.pone.0058933
Depositing User: Mary Creegan
Date Deposited: 18 Jul 2014 08:18
Last Modified: 06 Feb 2018 13:07
URI: https://archive.lstmed.ac.uk/id/eprint/3822

Statistics

View details

Actions (login required)

Edit Item Edit Item