Loss of pH control in Plasmodium falciparum parasites subjected to oxidative stress

van Schalkwyk, Donelly A., Saliba, Kevin J., Biagini, Giancarlo ORCID: https://orcid.org/0000-0001-6356-6595, Bray, Patrick and Kirk, Kiaran (2013) 'Loss of pH control in Plasmodium falciparum parasites subjected to oxidative stress'. PLoS ONE, Vol 8, Issue 3, e58933.

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Abstract

The intraerythrocytic malaria parasite is susceptible to oxidative stress and this may play a role in the mechanism of action of some antimalarial agents. Here we show that exposure of the intraerythrocytic malaria parasite to the oxidising agent hydrogen peroxide results in a fall in the intracellular ATP level and inhibition of the parasite's V-type H(+)-ATPase, causing a loss of pH control in both the parasite cytosol and the internal digestive vacuole. In contrast to the V-type H(+)-ATPase, the parasite's digestive vacuole H(+)-pyrophosphatase is insensitive to hydrogen peroxide-induced oxidative stress. This work provides insights into the effects of oxidative stress on the intraerythrocytic parasite, as well as providing an alternative possible explanation for a previous report that light-induced oxidative stress causes selective lysis of the parasite's digestive vacuole.

Item Type:	Article
Subjects:	QX Parasitology > Protozoa > QX 135 Plasmodia WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1371/journal.pone.0058933
Depositing User:	Mary Creegan
Date Deposited:	18 Jul 2014 08:18
Last Modified:	06 Feb 2018 13:07
URI:	https://archive.lstmed.ac.uk/id/eprint/3822

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