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PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination

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Pennington, Shaun ORCID: https://orcid.org/0000-0002-7160-6275, Thompson, Ameeka, Wright, Adam, Ferreira, Daniela ORCID: https://orcid.org/0000-0002-0594-0902, Wright, Angela, Faragher, Brian, Gordon, Stephen ORCID: https://orcid.org/0000-0001-6576-1116 and Gordon, M. A. (2012) 'PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination' in Digestive Disorders Federation Meeting, Liverpool, 17-20 June 2012.

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Official URL: http://gut.bmj.com/content/61/Suppl_2/A349.3

Abstract

Introduction: The human gut mucosal cellular response to oral vaccination has never been directly assessed. We studied the cognate cellular immune response to the live oral typhoid Ty21a vaccine in the gut mucosa of human volunteers, and compared it with that seen in peripheral blood.

Methods: 27 healthy volunteers were randomly assigned to a vaccinated (n=14) or a control (n=13) group for Ty21a typhoid vaccine. Peripheral blood was collected from all volunteers prior to vaccination and 18 days following immunisation or recruitment. Mucosal samples (15 jumbo biopsies from duodenum (n=25) ± colon (n=18)) were collected from all volunteers at gastroscopy +/− sigmoidoscopy on day 18. Mononuclear cells were isolated from mucosal tissue by disruption and collagenase digestion, and from blood by differential centrifugation. Cells were stimulated with Ty21a or control antigens, and stained for surface phenotype and intracellular cytokine production. Antigen-specific IFN-γ, TNF-α, and IL-2 production was determined

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	Poster abstract from Digestive Disorders Federation Meeting 17–20 June 2012, Liverpool
Subjects:	QU Biochemistry > Cells and Genetics > QU 300 General works QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 805 Vaccines. Antitoxins. Toxoids WC Communicable Diseases > Infection. Bacterial Infections > Enteric Infections > WC 270 Typhoid fever
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1136/gutjnl-2012-302514d.130
Depositing User:	Martin Chapman
Date Deposited:	05 Jan 2015 12:25
Last Modified:	26 Feb 2025 15:58
URI:	https://archive.lstmed.ac.uk/id/eprint/4696

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