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Poster Presentation: Development of monoclonal antibodies targeting the surface of Plasmodium falciparum-infected human erythrocytes

Alkurbi, Mohammed O., Higgins, M., Craig, Alister ORCID: and Pleass, Richard ORCID: (2013) 'Poster Presentation: Development of monoclonal antibodies targeting the surface of Plasmodium falciparum-infected human erythrocytes'. Tropical Medicine & International Health, Vol 18, Issue S1, p. 146.

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Cytoadhesion of Plasmodium falciparum-infected erythrocytes to endothelial cells lining the micro vessels of host organs is mainly linked to severe malaria infection. Cytoadhesion is mediated through the binding of P. falciparum erythrocyte membrane protein-1 (PfEMP-1) to various host receptors including Intercellular Adhesion Molecule 1 (ICAM-1), which has a role in cerebral malaria. In our study, we aim to develop monoclonal antibodies(mAbs) that target the potential adhesive Duffy Binding Like(DBL^a)domains of PfEMP-1 from ICAM-1 binding parasites.
mAbs were raised against four recombinant proteins namely rDBL13, rDBL27, rDBL31 and rDBL41. We developed thirteen distinct monoclonal and polyclonal hybridoma clones that secrete IgM-class antibodies capable of recognizing the immunizing rDBL^a proteins. Flow cytometry results indicated that at least five monoclonal and polyclonal antibodies significantly labelled the surface of erythrocytes infected with IT4var16, IT4var14, IT4var13 and IT4var1 strains. These data suggest that oligomeric IgM may be developed to interfere with the ability of parasitized erythrocytes (PEs) to sequester and thereby reduce the mortality rate of cerebral malaria infection.

Item Type: Article
Additional Information: Poster Presentation P. Special Issue: Abstracts of the 8th European Congress on Tropical Medicine and International Health & 5th Conference of the Scandinavian-Baltic Society for Parasitology, 10–13 September 2013, Copenhagen, Denmark
Subjects: QU Biochemistry > Cells and Genetics > QU 350 Cellular structures
QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies
QX Parasitology > Protozoa > QX 135 Plasmodia
WH Hemic and Lymphatic Systems > Hematologic Diseases. Immunologic Factors. Blood Banks > WH 150 Erythrocytes
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):
Depositing User: Lynn Roberts-Maloney
Date Deposited: 09 Feb 2015 10:21
Last Modified: 17 Jul 2019 14:14


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