A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans.

LaCourse, James ORCID: https://orcid.org/0000-0001-9261-7136, Perally, S., Hernandez-Viadel, M., Wright, H. A. and Brophy, P. M. (2008) 'A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans.'. Journal of Proteome Research, Vol 7, Issue 8, pp. 3314-3318.

Full text not available from this repository.

Abstract

Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations, protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.

Item Type:	Article
Subjects:	QU Biochemistry > QU 4 General works QX Parasitology > Helminths. Annelida > QX 203 Nematoda
Faculty: Department:	Groups (2002 - 2012) > Disease Control Strategy Group
Digital Object Identifer (DOI):	https://doi.org/10.1021/pr8001035
Depositing User:	Tina Bowers
Date Deposited:	01 Sep 2010 11:28
Last Modified:	06 Feb 2018 13:01
URI:	https://archive.lstmed.ac.uk/id/eprint/1387

Statistics

View details

Actions (login required)

Edit Item