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Diniz, M. R. V., Theakston, R.David G., Crampton, J. M., Cordeiro, M. D., Pimenta, A. M. C., De Lima, M. E. and Diniz, C. R. (2006) 'Functional expression and purification of recombinant Tx1, a sodium channel blocker neurotoxin from the venom of the Brazilian "armed" spider, Phoneutria nigriventer'. Protein Expression and Purification, Vol 50, Issue 1, pp. 18-24.
Full text not available from this repository.Abstract
Tx1 from the venom of the Brazilian spider, Phoneutria nigriventer, is a lethal neurotoxic polypeptide of M, 8600 Da with 14 cysteine residues. It is a novel sodium channel blocker which reversibly inhibits sodium currents in CHO cells expressing recombinant sodium (Nav1.2) channels. We cloned and expressed the Tx1 toxin as a thioredoxin fusion product in the cytoplasm of Escherichia coli. After semipurification by immobilized Ni-ion affinity chromatography, the recombinant Tx1 was purified by reverse phase chromatography and characterized. It displayed similar biochemical and pharmacological properties to the native toxin, and it should be useful for further investigation of structure-function relationship of Na channels. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | phoneutria nigriventer spider toxin sodium channel thioredoxin escherichia coli amino-acid-sequences calcium-channels escherichia-coli binding-sites toxin peptides proteins isomerization synaptosomes cloning |
| Subjects: | WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 420 Spiders. Scorpions. Centipedes. Leeches |
| Faculty: Department: | Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.pep.2006.06.012 |
| Depositing User: | Mary Creegan |
| Date Deposited: | 27 Sep 2011 13:32 |
| Last Modified: | 17 Jul 2020 10:57 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1476 |
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