van Beek, Lucille F., Surmann, Kristin, van den Berg van Saparoea, H. Bart, Houben, Diane, Jong, Wouter S. P., Hentschker, Christian, Ederveen, Thomas H. A., Mitsi, Elena, Ferreira, Daniela ORCID: https://orcid.org/0000-0002-0594-0902, van Opzeeland, Fred, van der Gaast – de Jongh, Christa E., Joosten, Irma, Völker, Uwe, Schmidt, Frank, Luirink, Joen, Diavatopoulos, Dimitri A. and de Jonge, Marien I. (2020) 'Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens'. Virulence, Vol 11, Issue 1, pp. 1310-1328.
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Elena Mitsi - Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens.docx - Accepted Version Available under License Creative Commons Attribution. Download (235kB) |
Abstract
Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae. Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.
Item Type: | Article |
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Subjects: | QW Microbiology and Immunology > Bacteria > QW 142 Gram-positive bacteria (General) QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 805 Vaccines. Antitoxins. Toxoids WC Communicable Diseases > Isolation and quarantine hospitals. Clinics, dispensaries, etc. Leper colonies > WC 100 General works WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 217 Pneumococcal infections |
Faculty: Department: | Clinical Sciences & International Health > Clinical Sciences Department |
Digital Object Identifer (DOI): | https://doi.org/10.1080/21505594.2020.1825908 |
Depositing User: | Rachael O'Donoghue |
Date Deposited: | 19 Nov 2020 12:23 |
Last Modified: | 09 Dec 2020 11:40 |
URI: | https://archive.lstmed.ac.uk/id/eprint/16140 |
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