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Wargo, A. R., Randle, Nadine, Chan, B. H. K., Thompson, J., Read, A. F. and Babiker, H. A. (2006) 'Plasmodium chabaudi: Reverse transcription PCR for the detection and quantification of transmission stage malaria parasites'. Experimental Parasitology, Vol 112, Issue 1, pp. 13-20.
Full text not available from this repository.Abstract
We have developed two reverse transcription polymerase chain reaction (RT-PCR) techniques to detect and quantify the transmission stages (gametocytes) of Plasmodium chabaudi malaria parasites. Both the qualitative and quantitative techniques are based on the amplification of mRNA coding for the P. chabaudi protein Pcs230, which is expressed exclusively in gametocytes. The quantitative RT-PCR (qRT-PCR) technique was developed and validated by examining serial dilutions of known gametocyte densities. The method generated a high correlation between calibration curves of blind samples (R-2 = 0.86). The technique was found to be specific, reproducible, and time efficient for quantification of both patent and sub-patent gametocytemia with a sensitivity level 100-1000 times greater than microscopy. The qualitative RT-PCR (RT-PCR) technique was used to monitor the persistence and dynamics of P. chabaudi gametocytes following acute infection. Mice in two independent experiments were sampled for up to 87 days post-infection. RT-PCR showed that gametocytes can persist for up to 8 weeks, post-infection, whereas microscopy could only detect gametocytes up to 6 weeks. Potential applications of the above techniques for studying the ecology, evolution, and epidemiology of malaria transmission are discussed. (c) 2005 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | plasmodium chabaudi gametocyte malaria transmission pcs230 chronic infection polymerase-chain-reaction time quantitative pcr falciparum gametocytes life-cycle unstable malaria eastern sudan infections expression genes gametocytogenesis |
| Subjects: | QX Parasitology > Protozoa > QX 135 Plasmodia QX Parasitology > QX 45 Host-parasite relations WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria |
| Faculty: Department: | Groups (2002 - 2012) > Vector Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.exppara.2005.08.013 |
| Depositing User: | Sarah Lewis-Newton |
| Date Deposited: | 27 May 2011 09:49 |
| Last Modified: | 06 Feb 2018 13:02 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1627 |
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