Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus

Grau-Bové, Xavie, Lucas, Eric ORCID: https://orcid.org/0000-0003-3892-1668, Pipini, Dimitra, Rippon, Emily, Van 't Hof, Arjen, Constant, Edi, Dadzie, Samuel, Egyir-Yawson, Alexander, Essandoh, John, Chabi, Joseph, Djogbenou, Luc, Harding, Nicholas J., Miles, Alistair, Kwiatkowski, Dominic, Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497 and Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388 (2021) 'Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus'. PLoS Genetics, Vol 17, Issue 1, e1009253.

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Abstract

Vector population control using insecticides is a key element of current strategies to prevent malaria transmission in Africa. The introduction of effective insecticides, such as the organophosphate pirimiphos-methyl, is essential to overcome the recurrent emergence of resistance driven by the highly diverse Anopheles genomes. Here, we use a population genomic approach to investigate the basis of pirimiphos-methyl resistance in the major malaria vectors Anopheles gambiae and A. coluzzii. A combination of copy number variation and a single non-synonymous substitution in the acetylcholinesterase gene, Ace1, provides the key resistance diagnostic in an A. coluzzii population from Côte d’Ivoire that we used for sequence-based association mapping, with replication in other West African populations. The Ace1 substitution and duplications occur on a unique resistance haplotype that evolved in A. gambiae and
introgressed into A. coluzzii, and is now common in West Africa primarily due to selection imposed by other organophosphate or carbamate insecticides. Our findings highlight the predictive value of this
complex resistance haplotype for phenotypic resistance and clarify its evolutionary history, providing tools to for molecular surveillance of the current and future effectiveness of pirimiphos-methyl based
interventions.

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1371/journal.pgen.1009253
Depositing User:	Stacy Murtagh
Date Deposited:	22 Jan 2021 12:21
Last Modified:	22 Jan 2021 12:21
URI:	https://archive.lstmed.ac.uk/id/eprint/16732

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