Role of the snake venom toxin jararhagin in proinflammatory pathogenesis: In vitro and in vivo gene expression analysis of the effects of the toxin

Gallagher, Paul, Bao, Yongde, Serrano, Solange M. T., Laing, Gavin, Theakston, R.David G., Guti, Jos, Escalante, Teresa, Zigrino, Paola, Moura-da-Silva, Ana M., Nischt, Roswitha, Mauch, Cornelia, Moskaluk, Christopher and Fox, Jay W. (2005) 'Role of the snake venom toxin jararhagin in proinflammatory pathogenesis: In vitro and in vivo gene expression analysis of the effects of the toxin'. Archives of Biochemistry and Biophysics, Vol 441, Issue 1, pp. 1-15.

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Abstract

To assess the indirect effects of snake venom metalloproteinases (SVMP) on host tissue local necrosis, we investigated the effect of the SVMP jararhagin on the gene expression profiles of human fibroblasts in vitro and mouse tissue in vivo. Two functional classes of up-regulated proteins, cell death and inflammatory disease were identified as being significantly populated. The changes in gene expression observed by qRT-PCR on laser microdissected mouse muscle tissue treated with jararhagin were similar with significant up-regulation of proinflammatory transcripts such as IL-1 beta, IL-6, CXCL1, CXCL2, IL-8, and apoptosis, inflammation responsive transcripts such as TNF-alpha induced protein 6. Proteolytically inactive jararhagin had no effect on the gene expression profile of fibroblasts, indicating proteolysis as the primary mechanism affecting gene expression of cells and tissues resulting in a proinflammatory, pro-apoptotic host response which likely exacerbates the local necrosis frequently observed at the site of envenoming. (C) 2005 Elsevier Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	snake venom metalloproteinases inflammation apoptosis anoikis gene expression necrosis-factor-alpha local tissue-damage bothrops-asper matrix metalloproteinases endothelial-cells induced apoptosis inflammation increments mechanisms cytokine
Subjects:	QU Biochemistry > Genetics > QU 450 General Works QZ Pathology > Manifestations of Disease > QZ 140 General manifestations of disease > QZ 150 Local reactions to injury and disease WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.abb.2005.06.007
Depositing User:	Ms Julia Martin
Date Deposited:	13 Sep 2011 09:39
Last Modified:	06 Feb 2018 13:02
URI:	https://archive.lstmed.ac.uk/id/eprint/1856

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