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Isolation and sequence analysis of P450 genes from a pyrethroid resistant colony of the major malaria vector Anopheles funestus

Amenya, D. A., Koekemoer, L. L., Vaughan, Ashley, Morgan, John, Brooke, B. D., Hunt, R. H., Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247, Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 and Coetzee, M. (2005) 'Isolation and sequence analysis of P450 genes from a pyrethroid resistant colony of the major malaria vector Anopheles funestus'. DNA Sequence, Vol 16, Issue 6, pp. 437-445.

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Abstract

Pyrethroid resistance has been demonstrated in populations of Anopheles funestus from South Africa and southern Mozambique. Resistance is associated with elevated P450 monooxygenase enzymes. In this study, degenerate primers based on conserved regions of Anopheles gambiae P450 CYP4, 6 and 9 families were used to amplify genomic and cDNA templates from A. funestus . A total of 12 CYP4, 12 CYP6 and 7 CYP9 partial genes have been isolated and sequenced. BLAST results revealed that A. funestus P450s generally have a high sequence identity to A. gambiae with above 75% identity at the amino acid level. The exception is CYP9J14. The A. gambiae P450 showing highest identity to CYP9J14 exhibits only 55% identity suggesting that CYP9J14 may have arisen from a recent duplication event. Molecular phylogenetic analysis based on amino acid sequences also supported this hypothesis. Intron positions, but not size, were highly conserved between the two species. The high level of orthology that exists in the P450 gene families of these two species may facilitate the prediction of individual P450 protein function.

Item Type: Article
Additional Information: Journal became Mitochondrial DNA in 2008
Uncontrolled Keywords: anopheles funestus pyrethroid resistance p450 orthologous genes gene duplication protein function polymerase-chain-reaction in-house fly insecticide resistance cytochromes p450 gambiae complex culicidae group diptera members identification cyp6d1
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QW Microbiology and Immunology > Immune Responses > QW 700 Infection. Mechanisms of infection and resistance.
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1080/10425170500356727
Depositing User: Sarah Lewis-Newton
Date Deposited: 30 Aug 2011 15:19
Last Modified: 30 Aug 2019 17:05
URI: https://archive.lstmed.ac.uk/id/eprint/1879

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