Detection of resistance-associated point mutations of organophosphate-insensitive acetylcholinesterase in the olive fruit fly, Bactrocera oleae (Gmelin)

Hawkes, Nicola J., Janes, R. W., Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 and Vontas, John (2005) 'Detection of resistance-associated point mutations of organophosphate-insensitive acetylcholinesterase in the olive fruit fly, Bactrocera oleae (Gmelin)'. Pesticide Biochemistry and Physiology, Vol 81, Issue 3, pp. 154-163.

Full text not available from this repository.

Abstract

We have recently identified two resistance-associated point mutations of organophosphate (OP)-insensitive acetylcholinesterase in the olive fruit fly Bactrocera oleae, the most important olive orchard pest world-wide. We have developed simple PCR-restriction fragment length polymorphism assays for each mutation, utilising an Accl restriction site created by Ile214Val, and a BssHII restriction site destroyed by a neutral change always accompanying the second mutation Gly488Ser. Samples from Greece homozygous for both mutations proved the most insensitive to dimethoate. The frequencies of these mutations in field-collected samples from several countries were investigated. Ninety-three percent of samples from Greece and Albania, where OPs have been extensively used in B. oleae control, were homozygous for both mutations. Resistance-associated alleles were detected at lower frequencies, but still with both mutations in conjunction in the majority of cases, in western Mediterranean countries with limited use of OPs. Samples from South Africa, however, did not have either of the resistance-associated mutations. The double mutation haplotype clearly confers a strong selective advantage in field populations of B. oleae exposed to OPs. (C) 2004 Elsevier Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	bactrocera oleae organophosphate acetylcholinesterase resistance mutations molecular diagnostic haplotype modelling drosophila-melanogaster acetylcholinesterase cdna-encoding acetylcholinesterase boophilus-microplus acari amino-acid substitution colorado potato beetle insecticide resistance torpedo-californica molecular-cloning mosquito vectors myzus-persicae
Subjects:	QU Biochemistry > Enzymes > QU 135 Enzymes QU Biochemistry > Genetics > QU 450 General Works WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.pestbp.2004.11.003
Depositing User:	Martin Chapman
Date Deposited:	06 Sep 2011 13:14
Last Modified:	06 Feb 2018 13:03
URI:	https://archive.lstmed.ac.uk/id/eprint/1948

Statistics

View details

Actions (login required)

Edit Item