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Salimi-Bejestani, M. R., McGarry, J.W., Felstead, S., Ortiz, P., Akca, A. and Williams, Diana J.L. (2005) 'Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test'. Research in Veterinary Science, Vol 78, Issue 2, pp. 177-181.
Full text not available from this repository.Abstract
An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | fasciola hepatica; cattle; immunodiagnosis; elisa linked-immunosorbent-assay; quantitative diagnosis; bovine fascioliasis; cattle; sheep infections; immunodiagnosis; immunoassay; challenge; responses |
| Subjects: | QX Parasitology > QX 4 General works |
| Faculty: Department: | Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008) |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.rvsc.2004.08.005 |
| Depositing User: | Ms Julia Martin |
| Date Deposited: | 05 Dec 2011 11:55 |
| Last Modified: | 06 Feb 2018 13:03 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/1957 |
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