Pan-African polyspecific antivenom produced by caprylic acid purification of horse IgG: an alternative to the antivenom crisis in Africa

Gutierrez, J. M., Rojas, E., Quesada, L., Leon, G., Nunez, J., Laing, Gavin, Sasa, M., Renjifo, J. M., Nasidi, A., Warrell, D. A., Theakston, R.David G. and Rojas, G. (2005) 'Pan-African polyspecific antivenom produced by caprylic acid purification of horse IgG: an alternative to the antivenom crisis in Africa'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 99, Issue 6, pp. 468-475.

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Abstract

A polyspecific Pan-African antivenom has been produced from the plasma of horses immunized with a mixture of the venoms of Echis ocellatus, Bitis arietans and Naja nigricollis, the three most medically important snakes in sub-Saharan Africa. The antivenom is a whole IgG preparation, obtained by caprylic acid precipitation of non-IgG plasma proteins. The antivenom effectively neutralizes the most important toxic activities of the three venoms used in the immunization in standard assays involving preincubation of venom and antivenom before testing. This antivenom compares favourably with other antivenoms designed for use in Africa with respect to neutralization of the toxins present in the venom of E. ocellatus. Caprylic acid fractionation of horse hyperimmune plasma is a simple, convenient and cheap protocol for the manufacture of high quality whole IgG antivenoms. It constitutes a potentially valuable technology for the alleviation of the critical shortage of antivenom in Africa. © 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	snake venom antivenom whole igg caprylic acid neutralization africa bothrops-asper venom polyvalent antivenom snake-venoms carpet viper bites neutralization fractionation hemorrhage colombia trial
Subjects:	WA Public Health > Preventive Medicine > WA 108 Preventive health services. Preventive medicine. Travel Medicine. WA Public Health > Accident and Injury Prevention. Disasters > WA 250 General works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 400 General works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.trstmh.2004.09.014
Depositing User:	Sarah Lewis-Newton
Date Deposited:	12 Dec 2011 18:35
Last Modified:	06 Feb 2018 13:03
URI:	https://archive.lstmed.ac.uk/id/eprint/1967

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