The RNA-Seq approach to studying the expression of mosquito mitochondrial genes

Neira-Oviedo, M., Tsyganov-Bodounov, A., Lycett, Gareth J. ORCID: https://orcid.org/0000-0002-2422-053X, Kokoza, V., Raikhel, A. S. and Krzywinski, Jaroslaw (2011) 'The RNA-Seq approach to studying the expression of mosquito mitochondrial genes'. Insect Molecular Biology, Vol 20, Issue 2, pp. 141-152.

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Abstract

In this study, we used extensive expressed sequence tag evidence obtained through 454 and Solexa next-generation sequencing to explore mtDNA transcription in male and female first instar larvae of Aedes aegypti and adults of Aedes aegypti, Anopheles gambiae, and Anopheles quadrimaculatus. Relative abundances of individual transcripts differed considerably within each sample, consistent with the differential stability of messenger RNA species. Large differences were also observed between species and between larval and adult stages; however, the male and female larval samples were remarkably similar. Quantitative PCR analysis of selected genes, cox1, l-rRNA and nd5, in larvae and adults of Ae. aegypti and in An. gambiae adults was consistent with the RNA-Seq-based quantification of expression. Finally, the absence of a conserved mtDNA region involved in transcriptional control in other dipterans suggests that mosquitoes have evolved a distinct mechanism of regulation of gene expression in the mitochondrion.

Item Type:	Article
Uncontrolled Keywords:	next-generation sequencing;mitochondrial genome;transcript abundance;transcription regulation
Subjects:	QU Biochemistry > Genetics > QU 450 General Works QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 525 Aedes
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1111/j.1365-2583.2010.01053.x
Depositing User:	Users 183 not found.
Date Deposited:	03 Jun 2011 08:44
Last Modified:	17 Jul 2020 10:58
URI:	https://archive.lstmed.ac.uk/id/eprint/2011

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