Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment

Tsakeng, Calmes Ursain Bouaka, Tanekou, Tito Tresor Melachio, Soffack, Steve Feudjio, Tirados, Inaki ORCID: https://orcid.org/0000-0002-9771-4880, Noutchih, Cedrique, Njiokou, Flobert, Bigoga, Jude Daiga and Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673 (2022) 'Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment'. Microorganisms, Vol 10, Issue 6, e1141.

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Abstract

The tsetse flies, biological vectors of African trypanosomes, harbour a variety of bacteria involved in their vector competence that may help in developing novel vector control tools. This study provides an inventory of tsetse bacterial communities in Cameroon and explores their possible associations with trypanosome establishment in Glossina palpalis palpalis. High throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene, with subsequent metagenomic, multivariate, and association analyses, were used to investigate the levels and patterns of microbial diversity in four tsetse species. Overall, 31 bacterial genera and four phyla were identified. The primary symbiont Wigglesworthia dominated almost all the samples, with an overall relative abundance of 47.29%, and seemed to be replaced by Serratia or Burkholderia in some G. tachinoides flies. Globally, significant differences were observed in the microbiome diversity and composition among tsetse species and between teneral and non-teneral flies, or between flies displaying or not displaying mature trypanosome infections. In addition, differential abundance testing showed some OTUs, or some bacteria taxa, associated with trypanosome maturation in tsetse flies. These bacteria could be further investigated for an understanding of their mechanism of action and alternatively, transformed and used to block trypanosome development in tsetse flies.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics. QX Parasitology > Insects. Other Parasites > QX 505 Diptera QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.3390/microorganisms10061141
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	08 Sep 2022 14:56
Last Modified:	14 Jun 2023 11:59
URI:	https://archive.lstmed.ac.uk/id/eprint/20515

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