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Ahmadi, Shirin, Pachis, Spyridon T., Kalogeropoulos, Konstantinos, McGeoghan, Farrell, Canbay, Vahap, Hall, Steven, Crittenden, Edouard, Dawson, Charlotte, Bartlett, Keirah, Gutiérrez, José María, Casewell, Nicholas 
ORCID: https://orcid.org/0000-0002-8035-4719, Keller, Ulrich auf dem and Laustsen, Andreas H.
(2022)
'Proteomics and histological assessment of an organotypic model of human skin following exposure to Naja nigricollis venom'. Toxicon, Vol 220, p. 106955.
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1-s2.0-S0041010122003063-main.pdf - Published Version Available under License Creative Commons Attribution. Download (9MB) | Preview |
Abstract
Snakebite envenoming was reintroduced as a Category A Neglected Tropical Disease by the World Health Organization in 2017. Since then, increased attention has been directed towards this affliction and towards the development of a deeper understanding of how snake venoms exert their toxic effects and how antivenoms can counter them. However, most of our in vivo generated knowledge stems from the use of animal models which do not always accurately reflect how the pathogenic effects of snake venoms manifest in humans. Moreover, animal experiments are associated with pain, distress, and eventually animal sacrifice due to the toxic nature of snake venoms. Related to this, the implementation of the 3Rs principle (Replacement, Reduction, and Refinement) in the use of experimental animals in snakebite envenoming research is recommended by the World Health Organization. Therefore, more humane experimental designs and new in vitro/ex vivo alternatives for experimental animals are sought after. Here, we report the use of an organotypic model of human skin to further elucidate the pathophysiology of the dermonecrotic effects caused by the venom of the black-necked spitting cobra, Naja nigricollis, in humans. The goal of this study is to expand the repertoire of available models that can be used to study the local tissue damages induced by cytotoxic venoms.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > Genetics > QU 460 Genomics. Proteomics QV Pharmacology > Toxicology > General Toxicology > QV 600 General works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles WR Dermatology > WR 20 Research (General) |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.toxicon.2022.106955 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 17 Nov 2022 14:16 |
| Last Modified: | 17 Nov 2022 14:16 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/21431 |
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