Flagellar attachment of Leishmania promastigotes to plastic film in vitro

Wakid, M. H. and Bates, Paul (2004) 'Flagellar attachment of Leishmania promastigotes to plastic film in vitro'. Experimental Parasitology, Vol 106, Issue 3-4, pp. 173-178.

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Abstract

Trypanosomatid parasites are able to use their flagella for attachment to cuticular surfaces within their arthropod hosts. In this study the attachment mechanism of Leishmania promastigotes was investigated using a new and quantifiable in vitro assay system. The results showed that hemidesmosomal flagellar attachment to three different plastic substrates occurred (Melinex, Polyvinyl, Thermanox). Attachment density was increased by scratching the surface of the substrate or by coating with the hydrocarbons n-octacosane and paraffin. Variation in attachment density was observed, depending on the culture medium and the parasite isolate used. All four species examined, L. braziliensis, L. donovani, L. major and L. mexicana, were capable of flagellar attachment in vitro. Collectively, these data indicate that flagellar attachment is mediated by a non-specific hydrophobic interaction in Leishmania species. (C) 2004 Elsevier Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	leishmania flagellum attachment promastigote trypanosoma-cruzi triatoma-infestans glossina-morsitans metacyclogenesis proboscis congolense mexicana differentiation epimastigotes cultivation
Subjects:	QX Parasitology > Protozoa > QX 50 Protozoa QX Parasitology > Protozoa > QX 70 Mastigophora. (e.g., Giardia. Trichomonas. Trypanosoma. Leishmania) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.exppara.2004.03.001
Depositing User:	Sarah Lewis-Newton
Date Deposited:	16 Feb 2012 15:31
Last Modified:	17 Jul 2020 10:57
URI:	https://archive.lstmed.ac.uk/id/eprint/2152

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