Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population

Greenland-Bews, Caitlin, Byrne, Rachel L., Owen, Sophie I. ORCID: https://orcid.org/0000-0002-0458-2357, Watkins, Rachel L., Bengey, Daisy, Buist, Kate, Clerkin, Karina, Escadafal, Camille, Finch, Lorna S., Gould, Susan, Giorgi, Emanuele, Hodgkinson, Andy, Mashenko, Larysa, Powell, Darren, Savage, Helen R., Thompson, Caitlin R., Turtle, Lance, Wardale, Jahanara, Wooding, Dominic, Edwards, Thomas, Atienzar, Ana Cubas and Adams, Emily R. ORCID: https://orcid.org/0000-0002-0816-2835 (2023) 'Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population'. BMC Infectious Diseases, Vol 23, Issue 1, e110.

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Abstract

Background: Rapid determination of an individual's antibody status can be beneficial in understanding an individual's immune response to SARS-CoV-2 and for initiation of therapies that are only deemed effective in sero-negative individuals. Antibody lateral flow tests (LFTs) have potential to address this need as a rapid, point of care test.

Methods: Here we present a proof-of-concept evaluation of eight LFT brands using sera from 95 vaccinated individuals to determine sensitivity for detecting vaccination generated antibodies. Samples were analysed on eight different brands of antibody LFT and an automated chemiluminescent microparticle immunoassay (CMIA) that identifies anti-spike antibodies which was used as our reference standard.

Results: All 95 (100%) participants tested positive for anti-spike antibodies by the chemiluminescent microparticle immunoassay (CMIA) reference standard post-dose two of their SARS-CoV-2 vaccine: BNT162b2 (Pfizer/BioNTech, n = 60), AZD1222 (AstraZeneca, n = 31), mRNA-1273 (Moderna, n = 2) and Undeclared Vaccine Brand (n = 2). Sensitivity increased from dose one to dose two in six out of eight LFTs with three tests achieving 100% sensitivity at dose two in detecting anti-spike antibodies.

Conclusions: These tests are demonstrated to be highly sensitive to detect raised antibody levels in vaccinated individuals. RDTs are low cost and rapid alternatives to ELISA based systems.

Item Type:	Article
Subjects:	QS Anatomy > QS 4 General works. Classify here works on regional anatomy QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 806 Vaccination
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1186/s12879-023-08033-1
Depositing User:	Marie Hatton
Date Deposited:	24 Mar 2023 11:26
Last Modified:	14 Jun 2023 10:09
URI:	https://archive.lstmed.ac.uk/id/eprint/22054

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