Bothrops asper metalloproteinase BaP1 is inhibited by alpha(2)-macroglobulin and mouse serum and does not induce systemic hemorrhage or coagulopathy

Escalante, T., Rucavado, A., Kamiguti, A. S., Theakston, R.David G. and Gutierrez, J. M. (2004) 'Bothrops asper metalloproteinase BaP1 is inhibited by alpha(2)-macroglobulin and mouse serum and does not induce systemic hemorrhage or coagulopathy'. Toxicon, Vol 43, Issue 2, pp. 213-217.

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Abstract

The ability of the P-I metalloproteinase BaP1, isolated from the venom of the snake Bothrops asper, to induce systemic bleeding, thrombocytopenia and defibrinogenation was assessed in an experimental mouse model. Intravenous administration of BaP1 caused neither systemic bleeding nor any evidence of pathology in lungs, kidneys, liver, heart and brain. Moreover, there were no alterations in the whole blood clotting time or in platelet numbers. In addition, BaP1 did not inhibit collagen-induced platelet aggregation in vitro. Proteolytic and hemorrhagic activities of BaP1 were readily inhibited by the plasma proteinase inhibitor, alpha2-macroglobulin, and normal mouse serum also inhibited hemorrhage. Such inhibition may explain why BaP1 induces multiple local tissue-damaging effects, but is largely devoid of systemic toxicity. (C) 2003 Elsevier Ltd. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	snake venom metalloproteinase systemic hemorrhage coagulopathy bothrops asper venom snake-venom metalloproteinases cysteine-rich domain platelet-aggregation human alpha-2-macroglobulin polyvalent antivenom jararaca venom jararhagin neutralization purification terciopelo
Subjects:	QV Pharmacology > Toxicology > General Toxicology > QV 600 General works QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 630 Toxins. Antitoxins QY Clinical Pathology > Blood. Blood Chemistry > QY 410 Coagulation WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 400 General works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.toxicon.2003.11.012
Depositing User:	Martin Chapman
Date Deposited:	19 Mar 2012 13:43
Last Modified:	06 Feb 2018 13:03
URI:	https://archive.lstmed.ac.uk/id/eprint/2227

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