LSTM Home > LSTM Research > LSTM Online Archive

Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques

Tools

Santus, Luisa, Sopena-Rios, Maria, García-Pérez, Raquel, Lin, Aaron E., Adams, Gordon C., Barnes, Kayla, Siddle, Katherine J., Wohl, Shirlee, Reverter, Ferran, Rinn, John L., Bennett, Richard S., Hensley, Lisa E., Sabeti, Pardis C. and Melé, Marta (2023) 'Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques'. Nature Communications, Vol 14, Issue 1, e3866.

Preview

Text
41467_2023_Article_39627.pdf - Published Version
Available under License Creative Commons Attribution.
Download (12MB) | Preview

Official URL: https://doi.org/10.1038/s41467-023-39627-7

Abstract

Long non-coding RNAs (lncRNAs) are involved in numerous biological processes and are pivotal mediators of the immune response, yet little is known about their properties at the single-cell level. Here, we generate a multi-tissue bulk RNAseq dataset from Ebola virus (EBOV) infected and not-infected rhesus macaques and identified 3979 novel lncRNAs. To profile lncRNA expression dynamics in immune circulating single-cells during EBOV infection, we design a metric, Upsilon, to estimate cell-type specificity. Our analysis reveals that lncRNAs are expressed in fewer cells than protein-coding genes, but they are not expressed at lower levels nor are they more cell-type specific when expressed in the same number of cells. In addition, we observe that lncRNAs exhibit similar changes in expression patterns to those of protein-coding genes during EBOV infection, and are often co-expressed with known immune regulators. A few lncRNAs change expression specifically upon EBOV entry in the cell. This study sheds light on the differential features of lncRNAs and protein-coding genes and paves the way for future single-cell lncRNA studies.

Item Type:

Article

Subjects:

WC Communicable Diseases > Virus Diseases > General Virus Diseases > WC 500 Virus diseases (General or not elsewhere classified)
WC Communicable Diseases > Virus Diseases > General RNA Virus Infections > WC 501 RNA virus infections (General or not elsewhere classified)

Repository link:

Item title	Item URI
Dataset for the article: Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques	https://archive.lstmed.ac.uk/id/eprint/22919

Faculty: Department:

Clinical Sciences & International Health > Clinical Sciences Department

Digital Object Identifer (DOI):

https://doi.org/10.1038/s41467-023-39627-7

SWORD Depositor:

JISC Pubrouter

Depositing User:

JISC Pubrouter

Date Deposited:

03 Jul 2023 08:22

Last Modified:

08 Aug 2023 10:22