Archer, John, Yeo, Shi Min, Gadd, Grace, Pennance, Tom, Cunningham, Lucas, Juhász, Alexandra, Jones, Sam, Chammudzi, Priscilla, Kapira, Donales R., Lally, David, Namacha, Gladys, Mainga, Bright, Makaula, Peter, LaCourse, James ORCID: https://orcid.org/0000-0001-9261-7136, Kayuni, Sekeleghe, Musaya, Janelisa, Stothard, Russell ORCID: https://orcid.org/0000-0002-9370-3420 and Webster, Bonnie L. (2024) 'Development, validation, and pilot application of a high throughput molecular xenomonitoring assay to detect Schistosoma mansoni and other trematode species within Biomphalaria freshwater snail hosts'. Current Research in Parasitology and Vector-Borne Diseases, Vol 5, p. 100174.
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Abstract
Schistosomiasis is a neglected tropical disease (NTD) caused by infection with parasitic trematodes of the genus Schistosoma that can lead to debilitating morbidity and mortality. The World Health Organization recommend molecular xenomonitoring of Biomphalaria spp. freshwater snail intermediate hosts of Schistosoma mansoni to identify highly focal intestinal schistosomiasis transmission sites and monitor disease transmission, particularly in low-endemicity areas. A standardised protocol to do this, however, is needed. Here, two previously published primer sets were selected to develop and validate a multiplex molecular xenomonitoring end-point PCR assay capable of detecting S. mansoni infections within individual Biomphalaria spp. missed by cercarial shedding. The assay proved highly sensitive and highly specific in detecting and amplifying S. mansoni DNA and also proved highly sensitive in detecting and amplifying non-S. mansoni trematode DNA. The optimised assay was then used to screen Biomphalaria spp. collected from a S. mansoni-endemic area for infection and successfully detected S. mansoni infections missed by cercarial shedding as well as infections with non-S. mansoni trematodes. The continued development and use of molecular xenomonitoring assays such as this will aid in improving disease control efforts, significantly reducing disease-related morbidities experienced by those in schistosomiasis-endemic areas.
Item Type: | Article |
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Subjects: | QX Parasitology > QX 20 Research (General) QX Parasitology > Helminths. Annelida > QX 353 Trematoda QX Parasitology > Helminths. Annelida > QX 355 Schistosoma QX Parasitology > QX 45 Host-parasite relations |
Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
Digital Object Identifer (DOI): | https://doi.org/10.1016/j.crpvbd.2024.100174 |
SWORD Depositor: | JISC Pubrouter |
Depositing User: | JISC Pubrouter |
Date Deposited: | 18 Apr 2024 08:21 |
Last Modified: | 18 Apr 2024 08:21 |
URI: | https://archive.lstmed.ac.uk/id/eprint/24315 |
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