Molecular cloning of phospholipases A(2) from venom glands of Echis carpet vipers

Bharati, K., Hasson, S.S., Oliver, J., Laing, Gavin, Theakston, R.David G. and Harrison, Robert (2003) 'Molecular cloning of phospholipases A(2) from venom glands of Echis carpet vipers'. Toxicon, Vol 41, Issue 8, pp. 941-947.

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Abstract

Venom toxin-specific antibodies offer a more rational treatment of snake envenoming than conventional antivenom. Here, we describe novel cDNAs encoding phospholipase A(2) (PLA(2)) isoforms from venom gland RNA of Echis pyramidum leakeyi (Epl), Echis sochureki (Es) and Echis ocellatus (Eo). The deduced amino acid sequences of these cDNAs encoded proteins with high overall sequence identity to the viper group II PLA(2) protein family, including the 14 cysteine residues capable of forming seven disulphide bonds that characterize this group of PLA(2) enzymes. Comparison of the PLA(2) sequences from Echis with those from related vipers failed to make significant geographic, taxonomic or PLA(2)-function distinctions between these Echis PLA(2) isoforms. However, their deduced hydrophilicity profiles revealed a conserved tertiary structure that we will exploit, by epidermal DNA immunization, to generate PLA(2)-neutralizing antibodies with polyspecific potential.

Item Type:	Article
Subjects:	QU Biochemistry > Enzymes > QU 136 Hydrolases QU Biochemistry > Genetics > QU 450 General Works WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI):	https://doi.org/10.1016/s0041-0101(02)00312-4
Depositing User:	Lynn Roberts-Maloney
Date Deposited:	08 Feb 2013 17:47
Last Modified:	06 Feb 2018 13:04
URI:	https://archive.lstmed.ac.uk/id/eprint/2500

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