A Synthetic Biology Approach to Transgene Expression in Insects

Leftwich, Philip T., Purcell, Jessica C., Anderson, Michelle A. E., Fragkoudis, Rennos, Basu, Sanjay, Lycett, Gareth ORCID: https://orcid.org/0000-0002-2422-053X and Alphey, Luke (2024) 'A Synthetic Biology Approach to Transgene Expression in Insects'. ACS Synthetic Biology, Vol 13, Issue 9, pp. 3041-3045.

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Abstract

The ability to control gene expression is pivotal in genetic engineering and synthetic biology. However, in most nonmodel and pest insect species, empirical evidence for predictable modulation of gene expression levels is lacking. This knowledge gap is critical for genetic control systems, particularly in mosquitoes, where transgenic methods offer novel routes for pest control. Commonly, the choice of RNA polymerase II promoter (Pol II) is the primary method for controlling gene expression, but the options are limited. To address this, we developed a systematic approach to characterize modifications in translation initiation sequences (TIS) and 3′ untranslated regions (UTR) of transgenes, enabling the creation of a toolbox for gene expression modulation in mosquitoes and potentially other insects. The approach demonstrated highly predictable gene expression changes across various cell lines and 5′ regulatory sequences, representing a significant advancement in mosquito synthetic biology gene expression tools.

Item Type:	Article
Subjects:	QU Biochemistry > Genetics > QU 500 Genetic phenomena QX Parasitology > Insects. Other Parasites > QX 500 Insects
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1021/acssynbio.4c00250
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	24 Sep 2024 14:24
Last Modified:	24 Sep 2024 14:24
URI:	https://archive.lstmed.ac.uk/id/eprint/25291

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