Improving molecular epidemiological surveillance of strongyloidiasis upon differentiation of Strongyloides fuelleborni fuelleborni from Strongyloides stercoralis

Cunningham, Lucas, Nevin, William, Verweij, Jaco J, Buonfrate, Dora, Scarso, Salvatore, Khieu, Virak, O'Ferrall, Angus M and Rollason, Sarah (2025) 'Improving molecular epidemiological surveillance of strongyloidiasis upon differentiation of Strongyloides fuelleborni fuelleborni from Strongyloides stercoralis'. Journal of Infectious Disease. (In Press)

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Abstract

Molecular epidemiological surveillance for zoonotic strongyloidiasis is confounded by a genus-specific TaqMan probe assay that conflates Strongyloides fuelleborni fuelleborni with Strongyloides stercoralis. To improve surveillance, we developed and validated a novel duplex species-specific TaqMan probe assay, screening a representative collection of available clinical samples. Our assay was highly discriminatory, evidencing no cross-reactivity, and had a lowest DNA detection limit of 1 pg/µL. However, as the genus-specific DNA assay has greater detection ability, we propose a 2-step protocol where samples are first screened with this assay then, if positive, screened with our species-specific assay, discriminating (co)infections between each threadworm species.

Item Type:	Article
Subjects:	QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA. QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1093/infdis/jiaf237
Depositing User:	Clare O'Neill
Date Deposited:	04 Jun 2025 11:14
Last Modified:	04 Jun 2025 11:14
URI:	https://archive.lstmed.ac.uk/id/eprint/26682

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