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Short Report: A Multiplex PCR Assay for Simultaneous Genotyping of kdr and ace-1 Loci in Anopheles gambiae

Kazanidou, A., Nikou, Dimitra, Grigoriou, M., Vontas, John and Skavdis, G. (2009) 'Short Report: A Multiplex PCR Assay for Simultaneous Genotyping of kdr and ace-1 Loci in Anopheles gambiae'. American Journal of Tropical Medicine and Hygiene, Vol 80, Issue 2, pp. 236-238.

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Abstract

The selection of insecticide-resistant genotypes in Anopheles gambiae, the most important malaria vector in Africa, makes disease control problematic in several endemic areas. The early detection and monitoring of resistance associated mutations in field mosquito populations is essential for the application of successful insecticide-based control interventions. Currently, the surveillance of these mutations is performed using individual assays, some of which require sophisticated and expensive equipment. Here we describe a novel multiplex polymerase chain reaction-based assay for detecting simultaneously the five single nucleotide polymorphisms in the voltage-gated sodium channel and the ace-l genes, which have been associated with the mosquito response to most commonly used insecticides.

Item Type: Article
Uncontrolled Keywords: knockdown resistance kdr insecticide resistance mosquito vectors mutation identification populations arabiensis east ddt
Subjects: WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
Faculty: Department: Groups (2002 - 2012) > Vector Group
Depositing User: Users 183 not found.
Date Deposited: 27 Apr 2010 15:33
Last Modified: 06 Feb 2018 12:59
URI: https://archive.lstmed.ac.uk/id/eprint/296

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