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Webster, Bonnie L, Culverwell, C Lorna, Khamis, I Simba, Mohammed, Khalfan A, Rollinson, David and Stothard, J Russell 
ORCID: https://orcid.org/0000-0002-9370-3420
(2013)
'DNA barcoding of Schistosoma haematobium on Zanzibar reveals substantial genetic diversity and two major phylogenetic groups.'. Acta Tropica, Vol 128, Issue 2, pp. 206-217.
Abstract
To shed light on the genetic diversity of Schistosoma haematobium on Zanzibar a DNA barcoding study was performed on parasite material isolated from different time-points 4 years apart. Substantive sequence variation was found within the mitochondrial cytochrome oxidase subunit I (cox1) and the NADH-dehydrogenase subunit 1 (nad1) with 27 and 22 unique haplotypes identified respectively and 38 when both gene regions were considered. Upon phylogenetic analysis and comparison with other S. haematobium isolates, haplotypes or barcode types partitioned into two discrete major groups, designated Group 1 and Group 2. Whilst Group 1 isolates were recovered from both Zanzibar and the African mainland, Group 2 isolates were exclusive to Zanzibar. A mixture of Group 1 and 2 parasites were recovered from individual children with no child shedding parasites of a single group haplotype alone. Whilst changes in general levels of genetic diversity between the two parasite isolation time-points were observed, no obvious change in genetic diversity was detected, despite large-scale drug distribution of praziquantel during the intervening period and there was no biased of Group 1 or 2 parasites persisting at the different time-points. To assist in future genetic screening of schistosome larval stages e.g. eggs, miracidia or cercariae, two new DNA-typing assays based on group-specific PCR primers and SNaPshot™ probes have been developed to distinguish Group 1 and 2 haplotypes.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > Genetics > QU 470 Genetic structures QU Biochemistry > Genetics > QU 500 Genetic phenomena QX Parasitology > Helminths. Annelida > QX 355 Schistosoma WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.actatropica.2012.06.002 |
| Depositing User: | Mary Creegan |
| Date Deposited: | 28 Oct 2013 15:02 |
| Last Modified: | 02 Dec 2019 12:52 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/3294 |
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