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The geographical distribution of allele polymorphisms of Plasmodium vivax in different regions of Thailand.

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Kosaisavee, Varakorn, Hastings, Ian ORCID: https://orcid.org/0000-0002-1332-742X, Craig, Alister ORCID: https://orcid.org/0000-0003-0914-6164 and Lek-Uthai, Usa (2012) 'The geographical distribution of allele polymorphisms of Plasmodium vivax in different regions of Thailand.'. Journal of the Medical Association of Thailand, Vol 95, Issue supp 6, S135-S140.

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Official URL: http://www.jmatonline.com/index.php/jmat/article/v...

Abstract

OBJECTIVE
To investigate the genetic structure of Plasmodium vivax circumsporozoite surface protein (PvCSP) and P. vivax merozoite surface protein 1 (PvMSP1) genes from field isolates of malaria parasites from four different regions of Thailand.
MATERIAL AND METHOD
The data was collected by cross-sectional survey, consisting of 273 P. vivax infected blood samples from malaria clinics in 4 different border regions of Thailand from February 2008 to February 2009. The dried blood spots were extracted for DNA and Plasmodium species confirmed by species-specific primer sets. PvCSP and PvMSP1 genes were amplified and their population genetics were analyzed by using the Heterozygosity (H) formula, F-STAT and LIAN programs.
RESULT
There was considerable variation in the PvMSP1 gene within 2 fragments for which HE was 0.8303, whereas PvCSP showed low HE at 0.1418. Significant differences in allele frequencies between sites were quantified by Fst, Linkage disequilibrium (LD). The results showed PvMSP1 F2; Fst = 0.063, p = 0.07; PvMSP1 F2 RFLP pattern; Fst = 0.154, p = 0.005; PvMSP1 F3; Fst = 0.23, p = 0.005 and the overall loci showed Fst = 0.151, p = 0.005 (Fisher's exact test). All values of Index association (I(S)A) were non-significant. There was no evidence of LD within the P. vivax populations.
CONCLUSION
H(E) at each locus of the PvMSP1 gene showed significant differences in allele frequencies between sites.

Item Type:	Article
Subjects:	QU Biochemistry > Genetics > QU 500 Genetic phenomena QX Parasitology > Protozoa > QX 135 Plasmodia WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries WB Practice of Medicine > Medical Climatology > WB 710 Diseases of geographic areas
Faculty: Department:	Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Depositing User:	Mary Creegan
Date Deposited:	10 Dec 2013 12:15
Last Modified:	19 Sep 2019 11:29
URI:	https://archive.lstmed.ac.uk/id/eprint/3393

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