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Nkhoma, Standwell, Nair, S., Mukaka, Mavuto, Molyneux, Malcolm E, Ward, Stephen 
ORCID: https://orcid.org/0000-0003-2331-3192 and Anderson, T. J. C.
(2009)
'Parasites bearing a single copy of the multi-drug resistance gene (pfmdr-1) with wild-type SNPs predominate amongst Plasmodium falciparum isolates from Malawi'. Acta Tropica, Vol 111, Issue 1, pp. 78-81.
Abstract
We genotyped 160 P. falciparum infections from Malawi for pfmdr-1 copy number changes and SNPs associated with in vivo tolerance and poor in vitro sensitivity to the component drugs of Coartem. We also measured in vitro susceptibility of 49 of these isolates to a variety of drugs in clinical use or with a potential for use in Africa. All 160 infections carried a single copy of pfmdr-1 but 34% exhibited sequence variation at 4 of the 5 polymorphic sites in pfmdr-1. Isolates carrying 86-Asn and 184-Tyr pfmdr-1 alleles were significantly less sensitive (p < 0.001) to mefloquine, lumefantrine, artemether and dihydroartemisinin compared with those bearing 86-Tyr and 184-Phe polymorphisms. This study provides baseline measures prior to policy change: continued surveillance for changes in baseline drug susceptibility, pfmdr-1 copy number and SNPs, and other putative Coartem resistance loci will be necessary to provide an early warning of emerging Coartem resistance in this setting. (C) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Additional Information: | Nkhoma, Standwell Nair, Shalini Mukaka, Mavuto Molyneux, Malcolm E. Ward, Stephen A. Anderson, Timothy J. C. |
| Uncontrolled Keywords: | Plasmodium falciparum; Antimalarial resistance; Multi-drug resistance gene (pfmdr-1); Copy number; Single nucleotide polymorphisms (SNPs); Artemisinin combination therapy (ACT); Coartem; Malawi |
| Subjects: | QX Parasitology > QX 20 Research (General) WB Practice of Medicine > Medical Climatology > WB 710 Diseases of geographic areas WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria WC Communicable Diseases > Tropical and Parasitic Diseases > WC 770 Therapy |
| Faculty: Department: | Groups (2002 - 2012) > Clinical Group Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.actatropica.2009.01.011 |
| Depositing User: | Mary Creegan |
| Date Deposited: | 27 Apr 2010 14:56 |
| Last Modified: | 17 Aug 2022 08:56 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/344 |
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