Tools
Tools
Zafra, R, Pérez-Écija, R A, Buffoni, L, Pacheco, I L, Martínez-Moreno, A, LaCourse, James 
ORCID: https://orcid.org/0000-0001-9261-7136, Perally, S, Brophy, P M and Pérez, J
(2013)
'Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica'. Research in Veterinary Science, Vol 94, Issue 3, pp. 602-609.
Abstract
Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and infected; group 3 (n=10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7-9 days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15 weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Glutathione transferase; Fasciola hepatica; Vaccine; Liver |
| Subjects: | QU Biochemistry > Proteins. Amino Acids. Peptides > QU 68 Peptides QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 805 Vaccines. Antitoxins. Toxoids QX Parasitology > Helminths. Annelida > QX 365 Fasciola WI Digestive System > WI 700 Liver |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.rvsc.2012.10.026 |
| Depositing User: | Mary Creegan |
| Date Deposited: | 17 Jul 2014 14:49 |
| Last Modified: | 06 Feb 2018 13:07 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/3805 |
Statistics
Actions (login required)
 |
Edit Item |