Prolonged gene knockdown in the tsetse fly Glossina by feeding double stranded RNA

Walshe, Deirdre ORCID: https://orcid.org/0000-0001-5531-2368, Lehane, Stella, Lehane, Mike and Haines, Lee ORCID: https://orcid.org/0000-0001-8821-6479 (2009) 'Prolonged gene knockdown in the tsetse fly Glossina by feeding double stranded RNA'. Insect Molecular Biology, Vol 18, Issue 1, pp. 11-19.

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Abstract

Reverse genetic studies based on RNA interference (RNAi) have revolutionized analysis of gene function in most insects. However the necessity of injecting double stranded RNA (dsRNA) inevitably compromises many investigations particularly those on immunity. Additionally, injection of tsetse flies often causes significant mortality. We demonstrate, at transcript and protein level, that delivering dsRNA in the bloodmeal to Glossina morsitans morsitans is as effective as injection in knockdown of the immunoresponsive midgut-expressed gene TsetseEP. However, feeding dsRNA fails to knockdown the fat body expressed transferrin gene, 2A192, previously shown to be silenced by dsRNA injection. Mortality rates of the dsRNA fed flies were significantly reduced compared to injected flies 14 days after treatment (Fed: 10.1% +/- 1.8%; injected: 37.9% +/- 3.6% (Mean +/- SEM)). This is the first demonstration in Diptera of gene knockdown by feeding and the first example of knockdown in a blood-sucking insect by including dsRNA in the bloodmeal.

Item Type:	Article
Uncontrolled Keywords:	rnai glossina dsrna feeding trypanosoma immunity morsitans-morsitans systemic rnai interference drosophila expression elegans dsrna trypanosomes glycoprotein challenge
Subjects:	QX Parasitology > Protozoa > QX 70 Mastigophora. (e.g., Giardia. Trichomonas. Trypanosoma. Leishmania) QW Microbiology and Immunology > Immunity by Type > QW 568 Cellular immunity. Immunologic cytotoxicity. Immunocompetence. Immunologic factors (General) QX Parasitology > Insects. Other Parasites > QX 505 Diptera QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics. QU Biochemistry > Genetics > QU 450 General Works
Digital Object Identifer (DOI):	https://doi.org/10.1111/j.1365-2583.2008.00839.x
Depositing User:	Users 183 not found.
Date Deposited:	10 Mar 2010 14:17
Last Modified:	17 Dec 2021 11:47
URI:	https://archive.lstmed.ac.uk/id/eprint/406

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