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Schmidt, Mark S., King, Christopher L., Thomsen, Edward 
ORCID: https://orcid.org/0000-0003-1136-6430, Siba, Peter M., Sanuku, Nelly and Fleckenstein, Lawrence
(2014)
'Liquid chromatography–mass spectrometry analysis of diethylcarbamazine in human plasma for clinical pharmacokinetic studies'. Journal of Pharmaceutical and Biomedical Analysis, Vol 98, pp. 307-310.
Abstract
A sensitive and selective liquid chromatographic method using mass spectrometric detection was developed for the determination of diethylcarbamazine (DEC) in human plasma. DEC and its stable isotope internal standard d3-DEC were extracted from 0.25 mL of human plasma using solid phase extraction. Chromatography was performed using a Phenomenex Synergi 4μ Fusion-RP column (2 mm × 250 mm) with gradient elution. The retention time was approximately 4.8 min. The assay was linear from 4 to 2200 ng/mL. Analysis of quality control samples at 12, 300, and 1700 ng/mL (N = 15) had interday coefficients of variation of 8.4%, 5.4%, and 6.2%, respectively (N = 15). Interday bias results were −2.2%, 6.0%, and 0.8%, respectively. Recovery of DEC from plasma ranged from 84.2% to 90.1%. The method was successfully applied to clinical samples from patients with lymphatic filariasis from a drug–drug interaction study between DEC and albendazole and/or ivermectin.
| Item Type: | Article |
|---|---|
| Subjects: | QU Biochemistry > QU 4 General works QV Pharmacology > QV 38 Drug action. QY Clinical Pathology > QY 25 Laboratory techniques and procedure WH Hemic and Lymphatic Systems > Hematologic Diseases. Immunologic Factors. Blood Banks > WH 400 Fluid elements. Plasma. Serum. Blood proteins. Blood protein disorders |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.jpba.2014.05.016 |
| Depositing User: | Lynn Roberts-Maloney |
| Date Deposited: | 16 Jan 2015 11:26 |
| Last Modified: | 06 Feb 2018 13:08 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/4762 |
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