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James, H.R., Kelly, L.A., Pochan, S.L., Commins, S.P., Workman, L.J., Nganga, L.W., Cooper, Philip and Platts-Mills, T.A.E. (2012) 'Parasite-related IgE Antibodies, Including IgE to Galactose-alpha-1,3-galactose, in Sera from Virginia and Ecuador'. Journal of Allergy and Clinical Immunology, Vol 129, Issue 2, AB82.
Full text not available from this repository.Abstract
Rationale
IgE antibodies to galactose-alpha-1,3-galactose (alpha-gal) have become increasingly prevalent in the southeastern United States. These antibodies have recently been linked to bites from the tick Amblyomma americanum, but the possibility of a role for other parasites remains.
Methods
Assays for IgE were performed on patients with delayed urticaria or anaphylaxis to meat and controls in Virginia (n=125), as well as a cohort from Esmeraldas Province, Ecuador (n=295) where Echinococcus is not endemic.
Results
The significant association between IgE antibodies to alpha-gal and IgE to Echinococcus was strongest in Virginia (r=0.74 vs r=0.62 for Ecuador, both p<0.001). Further, only 3/37 Echinococcus IgE-positive subjects in Virginia were not positive for IgE to alpha-gal. In Ecuador, 118/223 Ascaris-positive sera were negative for IgE to alpha-gal; by contrast, in Virginia 52/79 alpha-gal positive sera were negative for Ascaris and only one sera was Ascaris-positive and alpha-gal negative. Virginia sera with IgE to Echinococcus (class 2 and 3) were absorbed with alpha-gal linked to Sepharose beads, which removed all detectable IgE to Echinococcus.
Conclusions
We think that the limited number of positive assays for Echinococcus and Ascaris in Virginia can be explained by cross-reactivity with alpha-gal, which may also be relevant for Echinococcus in Esmeraldas. By contrast, many sera in Ecuador had high titer IgE to Ascaris with negative responses to alpha-gal. None of these IgE antibodies were significantly associated with asthma in the United States, while IgE to Ascaris was significantly associated with asthma in Ecuador.
| Item Type: | Article |
|---|---|
| Additional Information: | This article belongs to a special issue: Program and Abstracts of Papers to be Presented During Scientific Sessions: 2012 AAAAI Annual Meeting. |
| Subjects: | QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies QX Parasitology > QX 20 Research (General) |
| Faculty: Department: | Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group |
| Digital Object Identifer (DOI): | https://doi.org/10.1016/j.jaci.2011.12.631 |
| Depositing User: | Lynn Roberts-Maloney |
| Date Deposited: | 19 Jan 2015 11:34 |
| Last Modified: | 06 Feb 2018 13:08 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/4767 |
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