A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

Tamarozzi, Francesca, Wright, Helen L., Thomas, Huw B., Edwards, Steven W. and Taylor, Mark ORCID: https://orcid.org/0000-0003-3396-9275 (2014) 'A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry'. Immunology Letters, Vol 162, Issue 2 B, pp. 194-198.

Full text not available from this repository.

Abstract

We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Reference Works. General Immunology > QW 520 Research (General) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General) WH Hemic and Lymphatic Systems > Hematologic Diseases. Immunologic Factors. Blood Banks > WH 200 Leukocytes. Leukocyte disorders (General)
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.imlet.2014.10.025
Depositing User:	Martin Chapman
Date Deposited:	25 Feb 2015 15:35
Last Modified:	16 Sep 2019 09:01
URI:	https://archive.lstmed.ac.uk/id/eprint/4860

Statistics

View details

Actions (login required)

Edit Item