Characterization of an African trypanosome mutant refractory to lectin-induced death

Hackler, Amber L, Qiu, Yijian, Patrick, Stephen L, Lee, Soo Hee, Acosta Serrano, Alvaro ORCID: https://orcid.org/0000-0002-2576-7959 and Morris, James C (2015) 'Characterization of an African trypanosome mutant refractory to lectin-induced death'. Biochemistry and biophysics reports, Vol 4, pp. 33-38.

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Abstract

Incubation of African trypanosomes with the lectin concanavalin A (conA) leads to alteration in cellular DNA content, DNA degradation, and surface membrane blebbing. Here, we report the generation and characterization of a conA-refractory Trypanosoma brucei line. These insect stage parasites were resistant to conA killing, with a mediun lethal dose at least 50-fold greater than the parental line. Fluorescence-based experiments revealed that the resistant cells bound less lectin when compared to the parental line. Western blotting and mass spectrometry confirmed that the resistant line lacked an N-glycan required for conA binding on the cellular receptors, EP procyclin proteins. The failure to N-glycosylate the EP procyclins was not the consequence of altered N-glycan precursor biosynthesis, as another glycosylated protein (Fla1p) was normally modified. These findings support the likelihood that resistance to conA was a consequence of failure to bind the lectin trigger.

Item Type:	Article
Uncontrolled Keywords:	Trypanosoma brucei; concanavalin A; procyclins; surface molecule
Subjects:	QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Protozoa > QX 70 Mastigophora. (e.g., Giardia. Trichomonas. Trypanosoma. Leishmania) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.bbrep.2015.08.013
Depositing User:	Mary Creegan
Date Deposited:	16 Dec 2015 10:33
Last Modified:	14 Dec 2021 10:48
URI:	https://archive.lstmed.ac.uk/id/eprint/5419

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