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Alptekin, S, Bass, C, Nicholls, C, Paine, Mark 
ORCID: https://orcid.org/0000-0003-2061-7713, Clark, S J, Field, L and Moores, G D
(2016)
'Induced thiacloprid insensitivity in honeybees (Apis mellifera L.) is associated with up-regulation of detoxification genes'. Insect Molecular Biology, Vol 25, Issue 2, pp. 171-180.
Abstract
Honey bees, Apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme(s) involved remain to be characterized. In this work, a pretreatment of honey bees with a sublethal dose of thiacloprid resulted in induced insensitivity to the same compound immediately following thiacloprid feeding. A longer pretreatment time resulted in no, or increased, sensitivity. Transcriptome profiling, using microarrays, identified a number of genes encoding detoxification enzymes that were over-expressed significantly in insecticide-treated bees compared with untreated controls. These included five P450s, CYP6BE1, CYP305D1, CYP6AS5, CYP315A1, CYP301A1, and a carboxyl/cholinesterase (CCE) CCE8. Four of these P450s were functionally expressed in Escherichia coli and their ability to metabolize thiacloprid examined by liquid chromatography-mass spectrometry (LC-MS) analysis.
| Item Type: | Article |
|---|---|
| Subjects: | QW Microbiology and Immunology > Bacteria > QW 138 Enterobacteriaceae QX Parasitology > Insects. Other Parasites > QX 565 Hymenoptera (Bees. Wasps. Ants) WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) |
| Faculty: Department: | Biological Sciences > Vector Biology Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1111/imb.12211 |
| Depositing User: | Jessica Jones |
| Date Deposited: | 16 Feb 2016 14:40 |
| Last Modified: | 06 Feb 2018 13:11 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/5650 |
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