Herrera, M, Segura, A, Sanchez, A, Sanchez, A, Vargas, M, Harrison, Robert, Gutierrez, JM and Leon, G (2017) 'Freeze-dried EchiTAb+ICP antivenom formulated with sucrose is more resistant to thermal stress than the liquid formulation stabilized with sorbitol'. Toxicon, Vol 133, pp. 123-126.
Full text not available from this repository.Abstract
EchiTAb + ICP is a pan-African antivenom used for the treatment of snakebite envenomation in rural sub-Saharan African communities, where the cold chain can be difficult to maintain. To develop a formulation of EchiTAb + ICP that can be distributed and stored without refrigeration, we submitted three different formulations of EchiTAb + ICP: control (i.e. liquid antivenom formulated without stabilizer), liquid antivenom stabilized with sorbitol, and freeze-dried antivenom formulated with sucrose, to an accelerated stability study (i.e. 38 ± 2 °C and 75% relative humidity for 6 months). We analyzed changes in color, residual humidity, reconstitution time (for freeze-dried preparation), pH, osmolality, total protein concentration, antibody monomers content, turbidity, bacterial endotoxins, and pre-clinical neutralizing efficacy of the lethal effect of Echis ocellatus venom at 0, 3 and 6 months. In the control formulation, instability was evidenced by the development of a yellow coloration and an increment in aggregation and turbidity, without change in its neutralizing activity. The sorbitol-stabilized formulation did not develop marked aggregation or turbidity, but instability was evidenced by the development of yellow coloration and a drop in the neutralizing potency. The freeze-dried formulation maintained its neutralizing potency and did not show marked signs of instability, thus indicating that freeze-drying could confer EchiTAb + ICP with improved thermal stability required for distribution and storage at room temperature in sub-Saharan Africa.
Item Type: | Article |
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Additional Information: | Short Communication |
Subjects: | QU Biochemistry > QU 4 General works QW Microbiology and Immunology > Environmental Microbiology > QW 55 Environmental microbiology QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 573 Antigens QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 630 Toxins. Antitoxins WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries |
Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
Digital Object Identifer (DOI): | https://doi.org/10.1016/j.toxicon.2017.05.006 |
Depositing User: | Mary Creegan |
Date Deposited: | 19 Jun 2017 13:05 |
Last Modified: | 06 Feb 2018 13:14 |
URI: | https://archive.lstmed.ac.uk/id/eprint/7114 |
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