Susceptibility to three pyrethroids and detection of knockdown resistance mutation in Ghanaian Anopheles gambiae sensu stricto

Adasi, K. and Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 (2008) 'Susceptibility to three pyrethroids and detection of knockdown resistance mutation in Ghanaian Anopheles gambiae sensu stricto'. Journal of Vector Ecology, Vol 33, Issue 2, pp. 255-262.

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Abstract

Although insecticides play a crucial role in malaria control programs, this strategy is threatened by the development of resistance in the vectors to commonly used public health insecticides. Due to their known efficacy and lower toxicity to humans and non-target organisms, pyrethroids have been used in many vector control projects. In West Africa, pyrethroid resistance in the major malaria vector, Anopheles gambiae, had been reported in several countries. It was, therefore, imperative to investigate the susceptibility of this very important malaria vector in Ghana and characterize the resistance mechanism. Larvae of Anopheles gambiae sensu lato were collected from rural and urban sites and reared to adults. Species identification was by morphological characteristics, PCR, and HhaI restriction digest. Female mosquitoes that were two to three days old were selected and exposed to World Health Organization (WHO) diagnostic doses of three pyrethroids. A susceptible laboratory strain of An. gambiae sensu stricto was used as a reference. Both survivors and dead mosquitoes from bioassays were screened for the knockdown resistance mutation. An. gambiae sensu stricto was the only sibling species of the complex present in these localities with the molecular S form being predominant (>95%). Resistance to pyrethroids up to 8.5 fold was observed, with very high kdr frequency. The relative ease in using molecular techniques has resulted in rapid detection of ostensible insecticide resistance genes in malaria vectors. However, it is even more important to complement these molecular tools with routine insecticide testing in the field, especially if the insecticides are earmarked for public health use. Journal of Vector Ecology 33 (2): 255-262. 2008.

Item Type:	Article
Uncontrolled Keywords:	anopheles gambiae pyrethroid resistance south-eastern ghana insecticide resistance bed nets molecular-forms kdr mutation impregnated bednets combating malaria mosquito nets burkina-faso complex gene
Subjects:	WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors QU Biochemistry > Genetics > QU 470 Genetic structures QX Parasitology > Insects. Other Parasites > QX 515 Anopheles WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.3376/1081-1710-33.2.255
Depositing User:	Users 183 not found.
Date Deposited:	05 Jul 2010 13:28
Last Modified:	06 Feb 2018 13:00
URI:	https://archive.lstmed.ac.uk/id/eprint/714

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