Optimization of Mass-Rearing Methods for Anopheles arabiensis Larval Stages: Effects of Rearing Water Temperature and Larval Density on Mosquito Life-History Traits.

Mamai, Wadaka, Lobb, Leanne N, Bimbilé Somda, Nanwintoum S, Maiga, Hamidou, Yamada, Hanano, Lees, Rosemary ORCID: https://orcid.org/0000-0002-4232-9125, Bouyer, Jeremy and Gilles, Jeremie R L (2018) 'Optimization of Mass-Rearing Methods for Anopheles arabiensis Larval Stages: Effects of Rearing Water Temperature and Larval Density on Mosquito Life-History Traits.'. Journal of Economic Entomology, Vol 111, Issue 5, pp. 2383-2390.

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Abstract

Insect mass-rearing is an essential requirement for the sterile insect technique. Production at a large scale requires the development of standardized rearing procedures to produce good quality males able to compete with wild males to mate with wild females. Three sets of experiments (using trays placed on the table, the whole tray-rack system, and climate-controlled chambers) have been conducted aiming to determine the optimal water temperature and number of eggs to aliquot into each larval rearing tray to achieve the highest production of pupae. No difference was found in time to pupation, sex ratio, or male body size as a result of altering larval density. However, higher larval densities resulted in decreased emergence rate and female body size. A constant water temperature of 22°C delayed hatching and did not allow Anopheles arabiensis to complete larval development. Hatching eggs in water at 22°C and then increasing water temperature to 27°C resulted in decreased pupae production compared to eggs hatched and larvae maintained at a water temperature of 27°C throughout. Water temperature and larval density affected the production parameters of An. arabiensis mosquitoes, which has implications for mass release programs. We conclude that 4,000 eggs per 4 liter and a water temperature of 27°C were the optimal conditions for mass-rearing this mosquito species which yielded 105,000 pupae/larval rearing unit. These results are valuable information in the development of standard operation procedures for the efficient large-scale rearing of An. arabiensis mosquitoes.

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors QY Clinical Pathology > QY 25 Laboratory techniques and procedure QY Clinical Pathology > QY 4 General works
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1093/jee/toy213
SWORD Depositor:	JISC Pubrouter
Depositing User:	Stacy Murtagh
Date Deposited:	09 Aug 2018 10:49
Last Modified:	22 Aug 2019 11:08
URI:	https://archive.lstmed.ac.uk/id/eprint/9021

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