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Hempel, Benjamin-Florian, Damm, Maik, Petras, Daniel, Kazandjian, Taline D., Szentiks, Claudia A., Fritsch, Guido, Nebrich, Grit, Casewell, Nicholas 
ORCID: https://orcid.org/0000-0002-8035-4719, Klein, Oliver and Süssmuth, Roderich D.
(2023)
'Spatial Venomics─Cobra Venom System Reveals Spatial Differentiation of Snake Toxins by Mass Spectrometry Imaging'. Journal of Proteome Research, Vol 22, Issue 1, pp. 26-35.
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Casewell_1_Manuscript_JPR_Spatial_Venomics.pdf - Accepted Version Download (654kB) | Preview |
Abstract
Among venomous animals, toxic secretions have evolved as biochemical weapons associated with various highly specialized delivery systems on many occasions. Despite extensive research, there is still limited knowledge of the functional biology of most animal toxins, including their venom production and storage, as well as the morphological structures within sophisticated venom producing tissues that might underpin venom modulation. Here, we report on the spatial exploration of a snake venom gland system by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), in combination with standard proteotranscriptomic approaches, to enable in situ toxin mapping in spatial intensity maps across a venom gland sourced from the Egyptian cobra (Naja haje). MALDI-MSI toxin visualization on the elapid venom gland reveals a high spatial heterogeneity of different toxin classes at the proteoform level, which may be the result of physiological constraints on venom production and/or storage that reflects the potential for venom modulation under diverse stimuli.
| Item Type: | Article |
|---|---|
| Subjects: | QV Pharmacology > Toxicology > General Toxicology > QV 601 Antidotes and other therapeutic measures QV Pharmacology > Toxicology > General Toxicology > QV 602 Detection of poisons. Tests. Laboratory manuals. Technique WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles |
| Faculty: Department: | Biological Sciences > Department of Tropical Disease Biology |
| Digital Object Identifer (DOI): | https://doi.org/10.1021/acs.jproteome.2c00424 |
| SWORD Depositor: | JISC Pubrouter |
| Depositing User: | JISC Pubrouter |
| Date Deposited: | 15 Mar 2023 12:23 |
| Last Modified: | 15 Dec 2023 04:12 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/21782 |
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