Tools
Tools
Lo, Stephanie W, Gladstone, Rebecca A, van Tonder, Andries J, Hawkins, Paulina A, Kwambana-Adams, Brenda, Cornick, Jennifer, Madhi, Shabir A, Nzenze, Susan A, du Plessis, Mignon, Kandasamy, Rama, Carter, Philip E, Köseoglu Eser, Özgen, Ho, Pak Leung, Elmdaghri, Naima, Shakoor, Sadia, Clarke, Stuart C, Antonio, Martin, Everett, Dean, von Gottberg, Anne, Klugman, Keith P, McGee, Lesley, Breiman, Robert F and Bentley, Stephen D (2018) 'Global distribution of invasive serotype 35D Streptococcus pneumoniae post-PCV13 introduction'. Journal of Clinical Microbiology, Vol 56, Issue 7, e00228-18.
|
Text
JCM.00228-18.full.pdf - Accepted Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
A newly recognized pneumococcal serotype 35D, which differs from the 35B polysaccharide in structure and serology by not binding to factor serum 35a, was recently reported. The genetic basis for this distinctive serology is due to the presence of an inactivating mutation in , which encodes an O-acetyltransferase responsible for O-acetylation of a galactofuranose. Here, we assessed the genomic data of a worldwide pneumococcal collection to identify serotype 35D isolates and understand their geographical distribution, genetic background and invasiveness potential. Of 21,980 pneumococcal isolates, 444 were originally typed as serotype 35B by PneumoCaT. Analysis of revealed 23 isolates from carriage (n=4) and disease (n=19) with partial or complete loss-of-funtion mutations, including mutations resulting in pre-mature stop codons (n=22) and an in-frame mutation (n=1). These were selected for further analysis. The putative 35D isolates were geographically widespread and 65.2% (15/23) of them was recovered after PCV13 introduction. Compared with serotype 35B, putative serotype 35D isolates have higher invasive disease potentials based on odds ratio (OR) (11.58; 95% CI, 1.42-94.19 vs 0.61; 95% CI, 0.40-0.92) and a higher prevalence of macrolide resistance mediated by (26.1% vs 7.6%, p=0.009). Using Quellung, 50% (10/20) of viable isolates were serotype 35D, 25% (5/20) serotype 35B, and 25% (5/20) a mixture of 35B/35D. The discrepancy between phenotype and genotype requires further investigation. These findings illustrated a global distribution of an invasive serotype 35D among young children post-PCV13 introduction and underlined the invasive potential conferred by the loss of O-acetylation in the pneumococcal capsule.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NOT_LSTM |
| Subjects: | QW Microbiology and Immunology > Bacteria > QW 142 Gram-positive bacteria (General) QW Microbiology and Immunology > QW 4 General works. Classify here works on microbiology as a whole. WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 217 Pneumococcal infections |
| Faculty: Department: | Clinical Sciences & International Health > Malawi-Liverpool-Wellcome Programme (MLW) |
| Digital Object Identifer (DOI): | https://doi.org/10.1128/JCM.00228-18 |
| Depositing User: | Stacy Murtagh |
| Date Deposited: | 29 May 2018 11:27 |
| Last Modified: | 05 Nov 2024 08:18 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/8706 |
Statistics
Actions (login required)
 |
Edit Item |