Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging

Hamilton, Brett R, Marshall, David L, Casewell, Nicholas ORCID: https://orcid.org/0000-0002-8035-4719, Harrison, Robert, Blanksby, Stephen J and Undheim, Eivind Andreas Baste (2020) 'Mapping Enzyme Activity on Tissue by Functional Mass Spectrometry Imaging'. Angewandte Chemie (International ed. in English), Vol 59, Issue 10, pp. 3855-3858.

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Abstract

Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High-resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high-resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ .

Item Type:	Article
Subjects:	QY Clinical Pathology > QY 25 Laboratory techniques and procedure QY Clinical Pathology > QY 4 General works
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1002/anie.201911390
Depositing User:	Mary Creegan
Date Deposited:	07 Jan 2020 15:56
Last Modified:	15 May 2020 10:41
URI:	https://archive.lstmed.ac.uk/id/eprint/13471

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