Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes

Dyer, Naomi, Rebollo, E., Dominguez, P., Elkhatib, N., Chavrier, P., Daviet, L., Gonzalez, C. and Gonzalez-Gaitan, M. (2007) 'Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes'. Development, Vol 134, Issue 24, pp. 4437-4447.

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Abstract

The dramatic cell shape changes during cytokinesis require the interplay between microtubules and the actomyosin contractile ring, and addition of membrane to the plasma membrane. Numerous membrane-trafficking components localize to the central spindle during cytokinesis, but it is still unclear how this machinery is targeted there and how membrane trafficking is coordinated with cleavage furrow ingression. Here we use an arf6 null mutant to show that the endosomal GTPase ARF6 is required for cytokinesis in Drosophila spermatocytes. ARF6 is enriched on recycling endosomes at the central spindle, but it is required neither for central spindle nor actomyosin contractile ring assembly, nor for targeting of recycling endosomes to the central spindle. However, in arf6 mutants the cleavage furrow regresses because of a failure in rapid membrane addition to the plasma membrane. We propose that ARF6 promotes rapid recycling of endosomal membrane stores during cytokinesis, which is critical for rapid cleavage furrow ingression.

Item Type:	Article
Uncontrolled Keywords:	drosophila meiosis spermatogenesis testis adp-ribosylation factor rho-associated kinase plasma-membrane contractile ring phospholipase-d drosophila-melanogaster cleavage furrow chromaffin cells rab11 endosomes myosin-ii
Subjects:	QU Biochemistry > Cells and Genetics > QU 300 General works QX Parasitology > Insects. Other Parasites > QX 505 Diptera
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1242/dev.010983
Depositing User:	Ms Julia Martin
Date Deposited:	31 Aug 2010 11:29
Last Modified:	06 Feb 2018 13:01
URI:	https://archive.lstmed.ac.uk/id/eprint/1351

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