A novel association between clustered NF-kappa B and C/EBP binding sites is required for immune regulation of mosquito Defensin genes

Meredith, J. M., Munks, R. J. L., Grail, W., Hurd, H., Eggleston, P. and Lehane, Mike (2006) 'A novel association between clustered NF-kappa B and C/EBP binding sites is required for immune regulation of mosquito Defensin genes'. Insect Molecular Biology, Vol 15, Issue 4, pp. 393-401.

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Abstract

A comparative analysis identified key cis-acting regulatory elements responsible for the temporal control of mosquito Defensin gene expression. The promoters of Anopheles gambiae Defensin 1 and two isoforms of Aedes aegypti Defensin A are up-regulated by immune challenge. This stimulated activity depends upon a cluster of three NF-kappa B binding sites and closely associated C/EBP-like motifs, which function as a unit for optimal promoter activity. Binding of NF-kappa B and C/EBP like transcription factors is confirmed by electrophoretic mobility shift assay, including supershifts with antibodies to C/EBP. kappa B-like motifs are abundant within antimicrobial peptide gene promoters and most are very closely associated with putative C/EBP binding sites. This novel association between NF-kappa B and C/EBP binding sites may, therefore, be of widespread significance.

Item Type:	Article
Uncontrolled Keywords:	immune regulation defensin genes mosquito nf-kappa b c/ebp aedes-aegypti anopheles-gambiae insect immunity transcription factor drosophila immunity molecular-cloning vector mosquito diptericin promoter response element malaria vector
Subjects:	QU Biochemistry > Genetics > QU 475 Genetic processes QW Microbiology and Immunology > QW 4 General works. Classify here works on microbiology as a whole. QW Microbiology and Immunology > Immune Responses > QW 700 Infection. Mechanisms of infection and resistance. QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 515 Anopheles QX Parasitology > Insects. Other Parasites > QX 525 Aedes
Faculty: Department:	Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI):	https://doi.org/10.1111/j.1365-2583.2006.00635.x
Depositing User:	Sarah Lewis-Newton
Date Deposited:	21 Feb 2011 15:33
Last Modified:	06 Feb 2018 13:02
URI:	https://archive.lstmed.ac.uk/id/eprint/1553

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